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Cell Cycle Control: Mechanisms and Protocols (Methods in by Eishi Noguchi, Mariana C. Gadaleta

By Eishi Noguchi, Mariana C. Gadaleta

A selection of new experiences and protocols from best specialists in phone cycle regulation, telephone Cycle regulate: Mechanisms and Protocols, moment Edition offers a finished consultant to contemporary technical and theoretical developments within the box. starting with the overviews of assorted mobilephone cycle rules, this name provides the most up-tp-date protocols and state of the art strategies used to generate most recent findings in phone cycle legislation, akin to protocols to investigate mobile cycle occasions and molecules. Written within the winning Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, quite simply reproducible protocols, and notes on troubleshooting and fending off identified pitfalls.

Authoritative and simply accessible, telephone Cycle keep watch over: Mechanisms and Protocols, moment Edition should be a worthwhile source for a large viewers, starting from the skilled mobilephone cycle researchers trying to find new ways to the junior graduate scholars giving their first steps in phone cycle research.

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Extra resources for Cell Cycle Control: Mechanisms and Protocols (Methods in Molecular Biology)

Sample text

In yeast, several signaling pathways, including the PKA and TOR pathways, are proposed to mediate nutrient control of the cell cycle, and the unifying characteristic of these pathways is that they control ribosome biogenesis, such that translational activity serves as a cellular indicator of nutritional status. Another mechanism by which cells may coordinate cell size with cell cycle progression is via monitoring of cell geometry. The fission yeast S. pombe is shaped like a cylinder and grows lengthwise prior to division.

Activated Cds1/Chk2 then stabilizes the stalled replisome by phosphorylation of several subunits, notably the MCM helicase [59, 60]. In budding yeast, the Rad53 kinase serves the function of Cds1/Chk2. Like Cds1/Chk2, Rad53 has an N-terminal FHA domain followed by a kinase domain. However, Rad53 has an additional C-terminal FHA domain not seen in Cds1 that is important in its activation by DNA damage [61]. 4 S–M Dependency Upon stabilization, the replisome may stay in position until the blockade is removed or dNTPs restored.

Further, another kinase known as Dun1 is activated in budding Cell Cycle Checkpoints 33 yeast [52], which controls transcriptional responses to DNA damage including activation of ribonucleotide reductase, the enzyme required for dNTP synthesis. In higher organisms, the transcription factor p53 is a critical component of DNA damage checkpoints [25], particularly in G1 phase. p53 is regulated by a plethora of posttranslational modifications, including N-terminal phosphorylation on serine-15, which is catalyzed by ATR and its cousins ATM (Ataxia Telangiectasia Mutated) and DNA-PKcs (DNA-dependent protein kinase, catalytic subunit).

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